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KMID : 1377020210180040685
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Tissue Engineering and Regenerative Medicine
2021 Volume.18 No. 4 p.685 ~ p.691
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Adipose-Derived Stem Cell Exosomes Promoted Hair Regeneration
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Wu Jinyan
Yang Quyang
Wu Sainan
Yuan Ruoyue
Zhao Xiansheng
Li Yue
Wu Wenyu
Zhu Ningwen
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Abstract
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Background: Some scholars have found that dermal papilla spheroid?derived exosomes could promote the development of hair follicles. However, whether adipose-derived stem cell exosomes (ADSC-Exos) have a similar effect on hair growth has not been determined yet. Thus, the purpose of this article was to detect whether ADSC-Exos could promote hair regeneration.
Methods: Adipose-derived stem cells (ADSCs) were isolated from 6-week-old C57BL/6 mice. Then, ADSC-Exos were isolated from the ADSCs. Western blotting was used to detect specific exosome markers. The particle size and distribution of the exosomes were analyzed by NanoSight dynamic light scattering. A total of 12 nude mice were randomly divided into two groups (n?=?6 each): the ADSC-Exos group and the control group. For the control group, a mixture of freshly isolated dermal cells (DCs) and epidermal cells (ECs) was grafted. For the ADSC-Exos group, a mixture of DCs, ECs, and 50 μg/ml of ADSC-Exos was grafted. Gross evaluation of the hair regeneration was carried out 2?3 weeks after the transplantation, and the graft site was harvested for histology at the third week.
Results: The existence of exosomes derived from ADSCs was evidenced by CD63, ALX1, and CD9 expression. Two or three weeks after the grafting, the number of regenerated hairs in the ADSC-Exos group was higher than that in the control group (p?
Conclusion: Our results indicated that ADSC-Exos could promote in vivo hair follicle regeneration.
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KEYWORD
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Exosomes, Adipose-derived stem cells, Hair regeneration, Hair follicles
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